Structure/function studies of human decay-accelerating factor
نویسندگان
چکیده
منابع مشابه
Decay - Accelerating Factor - Release from Cell Membrane
Decay-accelerating factor (DAF) ~ is a membrane protein that inhibits amplification of the complement cascade on cell surfaces. By binding to C3b or C4b complement fragments that accidentally deposit on the cell membrane, DAF blocks the assembly of both the classical and alternative C3 and C5 convertases (1-3). In this manner, DAF protects host cells from damage by autologous complement. DAF is...
متن کاملCharacterization of the active sites in decay-accelerating factor.
Decay-accelerating factor (DAF) is a complement regulator that dissociates autologous C3 convertases, which assemble on self cell surfaces. Its activity resides in the last three of its four complement control protein repeats (CCP2-4). Previous modeling on the nuclear magnetic resonance structure of CCP15-16 in the serum C3 convertase regulator factor H proposed a positively charged surface are...
متن کاملDecay-accelerating factor modulates induction of T cell immunity
Decay-accelerating factor (Daf) dissociates C3/C5 convertases that assemble on host cells and thereby prevents complement activation on their surfaces. We demonstrate that during primary T cell activation, the absence of Daf on antigen-presenting cells (APCs) and on T cells enhances T cell proliferation and augments the induced frequency of effector cells. The effect is factor D- and, at least ...
متن کاملMolecular cloning of murine decay accelerating factor by immunoscreening.
Although the cDNA of human decay accelerating factor (DAF) which restricts complement activation on homologous cell membranes was cloned in 1987, all trials to detect the cDNA of mouse DAF by cross-hybridization were unsuccessful. However, by immunoscreening with a rabbit antiserum against purified mouse DAF, we successfully cloned the cDNA. It contains four typical short consensus repeats (SCR...
متن کاملMolecular and functional analysis of mouse decay accelerating factor (CD55).
Molecular cloning of mouse decay accelerating factor (DAF; CD55) predicted two forms of the molecule, one transmembrane (TM) and the other glycosylphosphatidylinositol (GPI)-anchored; these are encoded by separate genes termed Daf-GPI and Daf-TM. In the present study several additional isoforms of mouse DAF, generated by alternative splicing from these genes, are described. Northern-blot analys...
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ژورنال
عنوان ژورنال: Immunology
سال: 2000
ISSN: 0019-2805
DOI: 10.1046/j.1365-2567.2000.00086.x